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. 2021 Aug 21;18:35. doi: 10.1186/s12979-021-00245-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — B cells from young and elderly HIV individuals, as compared to those from healthy controls, express higher levels of mRNA of enzymes involved in metabolic pathways. B cells, isolated as described in Fig. 2, were left unstimulated. a The RNA was extracted and qPCR performed to evaluate RNA expression of LDHA (left) and PDHX (right). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. b PCA analyses with the axes showing the percentage of variation explained by PC1 and PC2. Each symbol corresponds to one individuals. Open circles, young healthy donors; filled circles, elderly healthy donors; open squares, young HIV + individuals; filled circles, elderly HIV + individuals