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. Author manuscript; available in PMC: 2022 Jul 1.
Published in final edited form as: Nat Chem Biol. 2021 May 6;17(7):806–816. doi: 10.1038/s41589-021-00788-5

Fig. 5: LiGAs measure affinity and avidity-based responses in cell-displayed GBPs.

Fig. 5:

a, Binding of LiGA-71 to construct hCD22-Fc normalized by binding of the same LiGA to protein-G beads, n=3. b, Binding of LiGA-71 to hCD22(+) CHO cells normalized by binding of the same LiGA to CHO cells, n=4. c, Binding of LiGA-71 to DC-SIGN(+) fibroblasts normalized by binding of the same LiGA to fibroblasts, n=4. d, Binding of LiGA 9×6 to DC-SIGN(+) fibroblasts performed analogously to (c), n=3. Phage virions that display 300 copies (d) or 460 copies (c) of core trimannoside exhibit significant binding to DC-SIGN clusters on the cell surface, virions with <150 copies or >1200 copies exhibit insignificant enrichment. Calculations of FC, FDR and error bars are identical to those described in Fig. 2h, Fig. 3a, and 4b–d.