Fig. 6:

a, Lethally irradiated mice were reconstituted with a 50:50 mixture of bone marrow from the two indicated recipient bone marrow to generate chimeric mice that have a mixture of hCD22⁺ and hCD22^- B cells both lacking mCD22 and with different CD45 congenic markers for sorting purposes. b, The LiGA was injected into mice via the tail vein. One hour later, the mice were euthanized, and organs collected. The B cells were stained with the appropriate antibodies and sorted for differential expression of hCD22. Sorted cells and cells from organs were analyzed for phage by PFU assay. c, Analysis of types of phage from the PFU assay expressed as PFU enrichment (top) or total PFU (bottom). Tissues from n=3 mice, p-values calculated by Student’s t-test. In c, Neu5Gcα2–6LacNAc and Neu5Acα2–6LacNAc were supplemented at ~1% ratio to simulate the contribution of a clone in a typical LiGA library.