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. 2021 Jun 28;10(6):bio058553. doi: 10.1242/bio.058553

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© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — The mtRNAPol- and mtTFB2- knockdown display reduced TFAM-nucleoid assemblies. EM images of Apex2-staining in the IFM of TFAM-Apex2 GAL4 control flies (A) and flies with mtRNAPol (B), mtTFB1 (C), and mtTFB2 (D) knockdown. Red arrowheads: TFAM-Apex2 signals. (E) The analysis of the staining intensities of TFAM-Apex2 relative to the control is plotted in box-and-whisker graphs. The t-test was performed and statistically significant differences between indicated groups are marked with asterisks (n=47-114; ***P<0.01 compared to the TFAM-Apex2 GAL4 control).