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. Author manuscript; available in PMC: 2022 Jul 23.
Published in final edited form as: J Mol Biol. 2021 May 24;433(15):167057. doi: 10.1016/j.jmb.2021.167057

Figure 2. Binding and peroxidase activation by PG and CL containing lipid vesicles.

Figure 2.

(a) Fraction of membrane-bound cyt c as a function of outer layer anionic lipid/cyt c ratio (“effective” anionic lipid/cyt c ratio). The molar fraction of PG or CL in the DOPG/DOPC and TOCL/DOPC vesicles is indicated. Saturation of cyt c binding to DOPG requires approximately twice the lipids as for TOCL. (b) Comparison of fractional oxygenation of polyunsaturated PG and CL by cyt c/H2O2 quantified by MS lipidomics. The molar ratio of PG to cyt c was kept at 25:1 and CL to cyt c at 12.5:1 so that the total phosphate to cyt c ratio was the same. The effective anionic lipid/cyt c ratio was 12.5 for PG and 6.3 for TOCL. The concentration of cyt c was kept at 1 μM, and H2O2 at 50 μM. Data are presented with mean value and SD.