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. Author manuscript; available in PMC: 2021 Aug 21.
Published in final edited form as: J Am Soc Mass Spectrom. 2021 Apr 5;32(6):1300–1311. doi: 10.1021/jasms.0c00451

Figure 1.

Figure 1.

Middle-down proteomics workflow for histone tail characterization. (A) Model system adopted in the current work. HeLa S3 cells were synchronized at S phase by double thymidine block, and M or G1 phase by thymidine nocodazole block, respectively. Asynchronized cells were also harvested. (B) MS-based proteomics was performed using the middle-down strategy. Histone H3.1 and H3.2 were purified by C18 separation, then cleaved by Glu-C. H3 N-terminal tails (AA 1–50) were separated through PGC column before high-resolution MS-MS/MS analysis.