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. 2021 Aug 13;2021:1394486. doi: 10.1155/2021/1394486

Figure 5.

Figure 5

Effects of MitoQ on the expression of Keap1 and Nrf2 in podocytes in vivo and in vitro. (a) Western blot analysis of Keap1 and Nrf2 in glomeruli from different groups, n = 3. (b) Representative immunohistochemistry staining of Nrf2 in glomeruli from different groups (original magnification, ×600), scale bar = 20 μm. (c) Representative fluorescence staining of Nrf2 (green), synaptopodin (podocytes, red), and DAPI (blue) in podocytes from different groups (original magnification, ×1000), scale bar = 10 μm, n = 5. (d) Quantitative analysis of protein levels from (a). (e) Western blot analysis of Keap1 and Nrf2 in podocytes in vitro, n = 3. (f) Representative fluorescence staining of Nrf2 in podocytes in vitro (original magnification, ×1000), scale bar = 15 μm, n = 5. (g, h) Quantitative analysis of protein levels from (e). (i) Quantitative analysis of the nuclear translocation of Nrf2. ∗P < 0.05 vs. control; #P < 0.05 vs. Ang II; $P < 0.05 vs. Ang II+MitoQ 50 nM.