Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 21;12(9):802. doi: 10.1038/s41419-021-03794-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 7 — A qRT-PCR revealed the influence of LiCl on circ_0026628 expression. Student’s t-test. B qRT-PCR verified the overexpression efficiency of SOX2 and the influence of upregulated SOX2 on SP1 and circ_0026628 expression. Student’s t-test. C qRT-PCR verified the knockdown efficiency of SOX2 and the influence of downregulated SOX2 on SP1 and circ_0026628 expression. One-way ANOVA. D JASPAR predicted the DNA-binding Motif of SOX2 and 2 binding sites of SOX2 on SP1 promoter. E ChIP assay revealed the enrichment of SP1 promoter in anti-SOX2 group relative to anti-IgG group. Student’s t-test. F Luciferase reporter assay unveiled the luciferase activity of reporters with wild-type or mutant SP1 promoter. Student’s t-test. *P < 0.05, **P < 0.01. “n.s.” indicates no significance.