Table 1.
Detection Limit of Three Molecular Diagnostic Assays for SARS-CoV-2 Nucleic Acid
| Molecular Diagnostic Assay | SARS-CoV-2 Target Region | % (No.) of Detected Replicates at the Indicated Dilution (Copies/mL) | ||||||
|---|---|---|---|---|---|---|---|---|
| 2000 | 1000 | 500 | 250 | 100 | 50 | 25 | ||
| BioFire RP2.1 | S, M | 100 (1/1) | 100 (1/1) | 100 (3/3) | 100 (10/10) | 20 (1/5) | 0 (0/1) | NA |
| Cobas Liat System | N, ORF1a/b | 100 (3/3) | 100 (6/6) | 100 (8/8) | 100 (10/10) | 70 (7/10) | 10 (1/10) | 0 (0/3) |
| Cephedi Xpert Xpress | N2, E | 100 (3/3) | 100 (6/6) | 100 (8/8) | 100 (10/10) | 50 (5/10) | 0 (0/10) | 0 (0/3) |
Abbreviations: S, spike; M, membrane proteins; N, nucleocapsid; E, envelope; ORF1 a/b, open reading frame 1 a/b.