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. 2021 Aug 17;14:4001–4017. doi: 10.2147/JIR.S324232

Figure 7.

Figure 7

The role of ADA2AR in macrophages secreting exosomes in BFM. (A) GW4869 had no obvious cytotoxicity. The cell-light EdU Apollo 488 in vitro flow cytometry Kit and PI was used to evaluate the cytotoxicity. GW4869 did not significant decrease the FITC high expressed cells and increase the PE high expressed cells. (B) Half violin graph showed no statistic difference between the two groups, n=5. (C) CGS21680 promoted macrophages secreting exosomes while ZM241385 inhibited. DiD (red cell membrane markers) was used to mark macrophages and DiO (green cell membrane markers) used to mark VECs. n=5. The contrast and brightness were evenly adjusted for each picture. Images ×400; original scale bar, 50 μm.