FIGURE 7.

Harvest of cells from microcarriers and characterization of harvested cells. A, Microscopic images of microcarriers before and after cell harvest. B, Proliferation of harvested cells in tissue culture flask. C, Flow cytometry analysis of surface markers of harvested cells. Red solid lines represent cells treated with negative and positive antibody cocktails and dashed lines represent cells treated with negative and positive isotype controls. D, Staining of adipogenic, osteogenic, and chondrogenic differentiation of harvested cells. Scale bar = 100 μm. E, The number of T cells per well after culturing PBMCs with different ratio of hMSCs (PBMCs:hMSCs = 100 to 1, 20 to 1, 8 to 1, 4 to 1, and 2 to 1). PBMCs cultured without hMSCs were used as a control group. Experiments were run in triplicate; Mean ± SD. **P < .001, Tukey post hoc tests after one‐way ANOVA