Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 9;148(15):dev199373. doi: 10.1242/dev.199373

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 2. — B cells are generated from mESC-derived Flk1⁺ progenitors in the absence of Notch signaling. (A) Experimental design. (B) Representative FACS analysis of CD11b⁺ myeloid and CD19⁺ B cells (gated as CD45⁺ cells) produced from Flk1⁺ cells on OP9 co-culture are shown. (C) The percentages of CD11b⁺ and CD19⁺ cells among CD45⁺ cells in the mESC culture with OP9 are shown (n=6). (D) Representative FACS analysis of donor-derived cells found in the peritoneal cavity of NSG mice transplanted with Rbpj^−/− mESC-derived B progenitors. (E) Percentage of donor-derived CD45⁺ cells in the recipient peritoneal cavity after Rbpj^+/−- and Rbpj^−/−-derived B-cell transplantation. (Rbpj^+/−, n=4; Rbpj^−/−, n=5). As a control, FL MNCs were transplanted into NSG mice (n=4). Individual data points are shown.