Fig. 3.

B-cell production from ECs in the E9.5 YS and P-Sp was not affected by Notch signaling inhibition. (A) Sorting strategy of CD31⁺VE-cad⁺ ECs from E9.5 YS and P-Sp from wild-type C57BL6 mice is shown. (B,C) Representative FACS analysis (B) and percentages of CD45⁺ cells expressing CD11b or CD19 (C) in the supernatant of ECs co-cultured on OP9 stromal cells with or without GSI (n=3). (D) Representative FACS analysis of E9.5 YS/P-Sp co-culture with OP9-DL1 with/without GSI (n=3 for each group). (E) Percentages of each blood lineage among mononuclear cells (MNCs) in the supernatant of E9.5 wild-type and VE-Cad-Cre: RbpjKO YS and P-Sp, co-culture with OP9 (n=3 for each group). Ter119/CD11b and CD19 expression were examined at day 6 and at day10 of OP9 co-culture, respectively. Student's unpaired t-test (*P<0.05). Data are mean±s.d.