Skip to main content
. 2021 Aug 19;9(8):e003031. doi: 10.1136/jitc-2021-003031

Figure 1.

Figure 1

Downregulated CBS expression was associated with poor prognosis, less apoptosis and more tumor-infiltrating Tregs in HCC. (A and B) Relative expression of CBS in HCC tumor versus peritumor tissues and the relationship between CBS expression and OS of HCC patients in TCGA (A) and GSE14520 (B) database, respectively. (C and D) The protein levels of CBS in 28 HCC tumor tissues and paired peritumor tissues were determined by western blot analysis. (E) The mRNA levels of CBS in 44 HCC tumor tissues and paired peritumor tissues were determined by real-time PCR analysis. (F) Representative IHC staining of CBS in HCC tumor tissue and matched non-tumor tissue. (G) The staining score of CBS in tumor tissues compared with peritumor tissues in all patients (n=118). (H) The staining score of CBS in tumor tissues from patients with early stage compared with those with late stage. (I) The relationship between CBS expression and the OS of HCC patients in Zhongshan cohort was determined by Kaplan-Meier analysis with log-rank test (n=236). (J and K) The correlation between CBS expression and the proportion of cleaved caspase-3 as well as the number of Tregs in HCC clinical samples (n=236) was analyzed by Pearson’s r test. In A, B, D, E, G and H, data are represented as mean±SEM, and statistical significance was calculated by Student’s two-tailed t-test. *P<0.05; ***p<0.001. CBS, cystathionine β-synthase; HCC, hepatocellular carcinoma; OS, overall survival.