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. 2021 Aug 19;9(8):e003031. doi: 10.1136/jitc-2021-003031

Figure 7.

Figure 7

miR-24–3p is an upstream suppressor of CBS. (A) The TargetScan database was used to show that miR-24–3p had sequences complementary to CBS 3’-UTR. (B) The correlation between CBS and miR-24-3p expression in HCC tumor tissues (n=24). (C) The expression level of miR-24-3p in HCC tumor tissues and paired adjacent peritumor specimens were investigated by real-time PCR (n=24). (D and E) MHCC97H and Hep3B cells were transfected with miR-24-3p or control mimics, then the expression of miR-24-3p was detected by real-time PCR (D), and the protein level of CBS was detected by western blot (E). (F) Complementary sequence between CBS 3’UTR and miR-24-3p, and the effect of miR-24-3p mimics or its inhibitor on the luciferase activity of wild or mutant CBS was tested by luciferase reporter assay. (G) Hypothetical scheme for the mechanisms of CBS inhibiting HCC growth. In B, data are represented as min-to-max bar graphs with median lines; in D–F, data are represented as mean±SEM, and statistical significance was calculated by Student’s two-tailed t-test (B, D–F). *P<0.05; **p<0.01; ***p<0.001. CBS, cystathionine β-synthase; HCC, hepatocellular carcinoma; NS, no significance.