. Author manuscript; available in PMC: 2022 Oct 1.

Published in final edited form as: Transl Res. 2021 May 23;236:87–108. doi: 10.1016/j.trsl.2021.05.003

Table V.

Classification of sensory neurons from adult mouse DRGs based on scRNA-seq clusters¹²⁹ and compared with previous marker-based classification

Neuron cluster based on transcriptional analysis and predicted function	Markers	Subtypes	Subtype markers	Classification of DRGs based on size, myelination, and markers——-Small neurons——Large neurons							Highly expressed TR channel types per cluster subtype
				PEPPeptidergic (myelinated+unmyelinated)	NPNonpeptidergic (unmyelinated)		TH (unmyelin-ated)C-LTMRsTh		NF(myelinated)
				Tac1	Calca	IB4	P2 × 3r	Plxnc1	Nefh	Ldhb
Small fiberneurons
C1	Gal	C1–1	Asic3^high								Trpv1^high
MHN	Adcyapl		Cldn9								Trpm8^hgh
		C1–2	Zcchcl2 Sstr2								Trpm3^high
C2	Nppb	02–1	Pvalb⁺								Trpv2^high
MHN (MI, IS)	Il31ra		S100b⁻
		C2–2	Pva1b⁻ S100b⁺								Trpm3^high
C3	Th		–								Trpv2^high
C-LTMR	Zfp521										Trpv4^high
C4	Mrgpra3	C4–1	Mrgprb4⁻ Ptpn6⁻
MHN (IS)	Rspo1	C4–2	Mrgprb4⁺ Ptpn6⁺
C5	Mrgprd (highly expressed)	C5–1	Gfra3⁺ Sstr2⁺								Trpm3^high
MHN (IS)	Prkcq, Lpar3	C5–2	Gfra3⁺ Sstr2⁺								Trpv2^high
C6	Mrgprd (moderately expressed) S100b	C6–1	Ntrk1⁺ Prkcq^low
MHN		C6–2	Ntrk1 Prkcq^high								Trpv2^high
Large fiber neurons
C7	Nxph1, Wnt7a, S100b	–	–
MHN (MS)
C8	Trappc31, Cgn11, S100b	C8–1	Ntrk3^high								Trpm8^high
MR			Htr1d^high
		C8–2	Ntrk1^high Htr3a^high								Trpm8^high
C9	Baiap2l1, Cadps2, S100b	C9–1	Asic3⁻ Cgnl1^low
MN		C9–2	Asic3⁺ Cgnl1^high
C10	Gal,	–	–								Trpv2^high
MR or P	Rspo1	–	–								Trpv1^high Trpa1^high Trpm3^high

Gray-filled squares indicate highly expressed markers per cluster.

Abbreviations: Adcyap1, pituitary adenylate cyclase-activating polypeptide 1; Asic3, acid sensing ion channel subunit 3; Baiap2l1, brain-specific angiogenesis inhibitor 1-associated protein 2-like protein 1; Cadps2, calcium-dependent secretion activator 2; Calca, calcitonin-related polypeptide-α; Cgnl1, cingulin-like 1; C-LTMR, C fiber low-threshold mechanoreceptor; Cldn9, claudin 9; Gal, galanin; Gfra3, GDNF family receptor-α3; Htr1d, 5-hydroxytryptamine receptor 1D; Il31ra, interleukin-31 receptor A; IS, itch-sensitive; Ldhb, lactate dehydrogenase B; Lpar3, lysophosphatidic acid receptor 3; MHN, mechanoheat nociceptor; MI, mechanically insensitive; MN, mechanical nociceptor; MR, mechanoreceptor; Mrgpra3, Mas-related G-protein coupled receptor member A3; Mrgprb4, Mas-related G-protein coupled receptor member B4; Mrgprd, Mas-related G-protein coupled receptor member D; MS, mechanically sensitive; Nefh, neurofilament heavy; NF, neurofilament-positive; NP, nonpeptidergic; Nppb, natriuretic peptide B; Ntrk1, neurotrophic receptor tyrosine kinase 1; Nxph1, neurexophilin 1; P, proprioceptor; Plxnc1, plexin C1; Ptpn6, protein tyrosine phosphatase non receptor type 6; Prkcq, protein kinase c theta; Pvalb, parvalbumin; P2 × 3r, purinergic receptor P2 × 3; PEP, peptidergic; Rspo1, R-spondin 1; Sstr2, somatostatin receptor 2; S100b, S100 calcium binding protein B; Tac1, tachykinin precursor 1; TH/Th, tyrosine hydroxylase; Trappc3l, trafficking protein particle complex subunit 3-like protein; Trpm, transient receptor potential melastatin; Trpv, transient receptor potential cation channel; Wnt7a, Wnt family member 7A; Zcchc12, zinc finger CCHC domain-containing protein 12; Zfp521, zinc finger protein 521.