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. Author manuscript; available in PMC: 2021 Nov 10.
Published in final edited form as: Mucosal Immunol. 2021 May 10;14(5):1133–1143. doi: 10.1038/s41385-021-00400-y

Figure 1. Eosinophil survival and surface marker expression in coculture.

Figure 1.

(A) The percentage of AnnexinV-/LiveDead- eosinophils was assessed by flow cytometry following monoculture or coculture (B) Eosinophil viability was assessed following 96 h of culture with media alone or coculture with varying quantities of EPC2 cells. (C) Surface expression of Siglec-8 was assessed at baseline and following 7 or 14 days of EPC2 coculture. (D) CD69 and (E) CCR3 expression were quantified on eosinophils at baseline or following 96 h of culture with IL-5 or GM-CSF or EPC2 coculture. Fold change (FC) in mean fluorescence intensity (MFI) was relative to control samples as measured by isotype or fluorescence minus one (FMO). Data shown are representative of at least 3 biological replicates, each with 3–4 technical replicates per condition and timepoint. Data shown are mean ± SD, and individual symbols represent technical replicates; bars signify p < 0.05.