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. Author manuscript; available in PMC: 2021 Nov 10.
Published in final edited form as: Mucosal Immunol. 2021 May 10;14(5):1133–1143. doi: 10.1038/s41385-021-00400-y

Figure 2. Contact-independent effect of coculture on eosinophil viability.

Figure 2.

(A) Eosinophil survival and (B) CD69 MFI were assessed on eosinophils cultured for 96 h with media or media with IL-5 or cocultured in the bottom well in contact with EPC2s (“C”) or in the transwell insert with EPC2s in the bottom well (“T”). (C) Eosinophil viability was measured after culture with media alone, media with GM-CSF, or dilutions of EPC2-conditioned media (CM); results were anayzed by ANOVA with Dunnett’s post-test, and bars indicate p < 0.05. Data are representative of 3 biological replicates, each with 3–4 technical replicates per condition. In A-C, data shown are mean ± SD, and individual symbols represent technical replicates.