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. Author manuscript; available in PMC: 2021 Nov 10.
Published in final edited form as: Mucosal Immunol. 2021 May 10;14(5):1133–1143. doi: 10.1038/s41385-021-00400-y

Figure 3. Soluble factors observed in eosinophil and epithelial cell coculture.

Figure 3.

(A) A 65-analyte multiplex array was used to identify inflammatory mediators in culture supernatants from 7 days of IL-5-treated eosinophil monocultures, cocultures, cocultures with IL-13, EPC2 monocultures with EPC2 monocultures with IL-13. Values within the heatmap represent the mean concentration (pg/mL) for each treatment group. (B) Individual analytes are plotted. Significant differences were assessed with one-way ANOVA and Tukey post-test -bars indicate p < 0.01. Samples from three biological replicates were analyzed, with 2–3 technical replicates of each culture condition for each biological replicate. Representative data shown are mean ± SD, and individual symbols represent technical replicates.