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. Author manuscript; available in PMC: 2021 Nov 10.
Published in final edited form as: Mucosal Immunol. 2021 May 10;14(5):1133–1143. doi: 10.1038/s41385-021-00400-y

Figure 4. Functional impact of soluble factors in coculture on eosinophil migration and viability.

Figure 4.

(A) Eosinophils cultured for 24 h with media alone or media with IL-5 were seeded in porous inserts and allowed to migrate for 4 h in response to media, EPC2-conditioned media (CM), IL-13–treated EPC2–conditioned media or CCL26. (B) Viability was assessed after 14 days of culture with media alone or media with IL-5 or IL-13 or EPC2 coculture with or without IL-13. (C) Eosinophil viability was measured by Annexin-V staining and viability dye after 96 h of culture with either IL-5, GM-CSF, or EPC2-conditioned media (CM) with neutralizing antibody against GM-CSF or IgG control (Isotype). Data are representative of experiments that were repeated 2–3 times with 3 technical replicates per condition. Data shown are mean ± SEM, and individual symbols represent technical replicates. Significant differences were assessed with one-way ANOVA and Tukey post-test. Bars indicate p < 0.01, ** p < 0.001. ND, not detected.