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. Author manuscript; available in PMC: 2022 Aug 19.
Published in final edited form as: Cell Chem Biol. 2021 Mar 8;28(8):1145–1157.e6. doi: 10.1016/j.chembiol.2021.02.007

Figure 5.

Figure 5.

NSC 194308 alters splicing of U2AF2-sensitive transcripts in HEK 293T cells. (A - C) qRT-PCR of gene transcripts with known responsiveness to U2AF2 family members. NSC 194308-treated samples (blue, left) are compared with samples treated with U2AF2-targeted Stealth™ siRNAs (or control siRNA) for either two days (magenta, center) or three days (purple, right). The products of the indicated gene transcripts were normalized to GAPDH controls. The qRT-PCR results are mean ± SD of three reactions. (D - F) RT-PCR of the indicated U2AF2-regulated gene transcripts following either treatment with NSC 194308 (left) or U2AF2 knockdown (right). A mocked-treated control (0.1% v/v DMSO) matches the NSC 194308 solvent. The mean ± SD exon-skipped:included ratios of background-corrected bands from three reactions (six for DMSO control) are plotted below representative ethidium-bromide-stained agarose gels. The expected PCR products are schematized (right). Two-tailed, unpaired t-tests with Welch’s correction: *, p < 0.05; **, p < 0.005; ***, p < 0.0005; ****, p < 0.00005. Immunoblots are shown in Figure S3.