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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Gastroenterology. 2021 Jun 4;161(3):924–939.e11. doi: 10.1053/j.gastro.2021.05.057

Figure 6. Novel BE marker ANXA10 and co-expression of gastric and intestinal features.

Figure 6.

(A) scATAC-seq identified open chromatin at ANXA10, a stomach-restricted locus, in BE. Cis-element activity is confirmed by H3K4me2 FIT-seq on 11 independent BE samples.

(B) Representative ANXA10 immunostaining of 5 independent BE samples shows regions of prominent expression varying within and between metaplastic glands.

(C-D) Two-color immunohistochemistry for CDX2 and stomach- (MUC5AC) or intestine- (MUC2) specific genes in sequential tissue sections from 5 different representative BE samples (panels C and D represent specimens from different patients). MUC5AC is co-expressed with MUC2 and CDX2 in hundreds, but not all, single cells. Adjoining squamous (Sq.) mucosa lacks all 3 markers. Insets show enlarged views of the boxed regions, highlighting marker co-expression.