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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Gastroenterology. 2021 Jun 4;161(3):924–939.e11. doi: 10.1053/j.gastro.2021.05.057

Fig. 7. Heterogeneous cell identities in BE and gastric intestinal metaplasia.

Fig. 7.

(A-C) Intra-sample heterogeneity of co-existing stomach and intestinal states in BE. Panel A shows representative areas of CDX2- (asterisks) and CDX2+ areas within presumptive clonal BE glands. CDX2 co-expression with additional stomach markers, CLDN18 (B) and TFF1 (C), is evident in specimens (n=5) also used for ANXA10 immunostaining (Fig. 6B). Two representative specimens are shown; middle panels in B and C show boxed areas in the left panels at higher magnification. Both markers reveal extensive intra- and inter-gland heterogeneity. Images were captured through 20X or 40X objectives. Scale bar is shown in D, left panel.

(D) Gastric intestinal metaplasia also harbors dual and heterogeneous gastric and intestinal cell states. In these representative images (n=7, additional examples in Fig. S6C–D), the superficial epithelium expresses CDX2 but many deep glands do not (asterisks). MUC5AC is present broadly, but only CDX2+ cells express MUC2. Insets show boxed areas at higher magnification.