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Quantitative real-time PCR analysis of c-Fos, cdk5, p35, nicotinic subtype receptors and serotonergic signalling pathway genes evaluated 1 h after the test on day 7 and 60 after nicotine cessation. Data are mean ± SEM of 5 zebrafish per group. *p < 0.05, **p < 0.01 compared to vehicle group (one-way ANOVA followed by Tukey’s test).
