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. Author manuscript; available in PMC: 2022 Sep 1.
Published in final edited form as: Gastroenterology. 2021 Jun 8;161(3):953–967.e15. doi: 10.1053/j.gastro.2021.05.058

Figure 3. Inhibition of SPEM in Wfdc2-knockout (KO) mice after DMP-777 treatment.

Figure 3.

(A) Immunohistochemistry images for TFF2. Scale bars, 40 μm. (B) Quantification of TFF2+ cells in single glands (n = 5 per group, two-tailed unpaired Student’s t-test, ***p < 0.001). (C) TEM images of chief cells. Scale bars, 5000 nm. ZG, zymogen granule. (D) Immunofluorescence images for GSII, and GIF in wild-type (WT) and KO mice. Scale bars, 20 μm. (E) Comparison of SPEM cells during DMP-777 treatment. Each graph represents the number of positive cells in single glands (n = 5 per group, two-tailed unpaired Student’s t-test, **p < 0.01). (F) Immunofluorescence images for GSII, and MKI67. Scale bars, 20 μm. (G) Comparison of proliferative SPEM cells during DMP-777 treatment. Each graph represents the number of positive cells in single glands (n = 5 per group, two-tailed unpaired Student’s t-test, **p < 0.01, ***p < 0.001). (H) Immunofluorescence images for GSII, and CD44v9 of the Unt, DMP-777 (D7)-treated, and HDT-treated corpus. Scale bars, 100 μm. (I) RT-qPCR for Cftr and Gpx2 in the Unt, DMP-777 (D7)-treated, and HDT-treated corpus. mRNA expression levels were normalized by Gapdh (n = 5–7 per group, two-tailed unpaired Student’s t-test, *p < 0.05). All data are presented as a mean ± SEM. Unt, Untreated; D7, 7 days of DMP-777 treatment; D14, 14 days of DMP-777 treatment; R14, 14 days of recovery; HDT, high-dose tamoxifen.