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. Author manuscript; available in PMC: 2021 Dec 28.
Published in final edited form as: Mucosal Immunol. 2021 Jun 28;14(5):1088–1099. doi: 10.1038/s41385-021-00427-1

Figure 4.

Figure 4.

Granzyme B-deficient CD4+ T cells activated in vivo display a distinct gene expression profile. 1×105 CD4+CD45RBhi T cells from WT or Gzmb−/− donor mice were adoptively transferred into Rag2−/− recipient mice. Three weeks after transfer, donor-derived cells were recovered from the MLN, sorted for live TCRβ+CD4+, their RNA isolated and sequenced. (a) Representative heat map. (b) Representative gene set enrichment analysis. The green line on the plots indicate the enrichment score; the black lines show where the genes related to the pathway are located in the ranking; the legend at the bottom indicates whether the expression of the genes correlate more with CD4+ T cells derived from WT or Gzmb−/− mice. (c) Real-time PCR validation of selected genes. For day 0, n=2; for day 21, n=3–6. *P<0.05; **P<0.01. Student’s t-Test.