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. 2021 Aug 5;6(4):300–314. doi: 10.1089/can.2020.0046

FIG. 3.

FIG. 3.

Histologic scoring. Histologic scores using our novel scoring system were determined on spinal cords for WT/SAL (n=13), WT/EAE (n=14), Cnr1−/−/SAL (n=13), and Cnr1−/−/EAE (n=12) mice (A). No infiltration was seen in any of the SAL mice examined, so scores for Cnr1−/−/SAL and WT/SAL are all 0. Histologic scores for each spinal cord section were analyzed using the Mann–Whitney U-test to compare between groups. To validate this method of scoring, the histologic scores were correlated to the clinical scores for each mouse (B). Lesion area for each mouse was also tabulated by finding the lesion area for each of the five spinal cord sections and adding them together, and then, this area was correlated to the clinical score for each mouse to determine if the lesion area was more representative of clinical disease (C). Error bars represent the SD for each group. *p<0.05 difference between WT and Cnr1−/− in EAE.