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. 2021 Aug 5;6(4):300–314. doi: 10.1089/can.2020.0046

FIG. 4.

FIG. 4.

IFN-γ and IL-17A in serum and MOG35–55 restimulation cultures. Splenocytes were harvested from EAE and SAL control mice restimulated with MOG35–55 peptide for 3 days. The supernatants from these cultures were then tested by ELISA for concentrations of IFN-γ (WT/SAL [n=11], WT/EAE [n=11], Cnr1−/−/SAL [n=11], and Cnr1−/−/EAE [n=12]), (A) and IL-17A (WT/SAL [n=11], WT/EAE [n=14], Cnr1−/−/SAL [n=11], and Cnr1−/−/EAE [n=12]) (B). Serum was also taken from each mouse at the time of necropsy and tested by ELISA for concentrations of IFN-γ (WT/SAL [n=13], WT/EAE [n=13], Cnr1−/−/SAL [n=12], and Cnr1−/−/EAE [n=10]), (C) and IL-17A (WT/SAL [n=10], WT/EAE [n=14], Cnr1−/−/SAL [n=11], and Cnr1−/−/EAE [n=12]) (D). Statistical analysis was performed using two-way ANOVA followed by Sidak's post hoc test. Bars represent the mean±SD for each group. *p<0.05 difference between WT and Cnr1−/− in EAE. ELISA, enzyme-linked immunosorbent assay; MOG, myelin oligodendrocyte glycoprotein.