Fig. 7.

(A) SDS-PAGE of the in vitro digestion products of A. colubrina vicillin by the homogenate of C. maculatus larvae intestinal enzymes: M - molecular mass marker; A. colubrina vicillin; IH - Intestinal homogenate of larvae; (0 to 24), time of exposure of AcV, 30 mg, to intestinal homogenate of larvae in hours in vitro. (B) elution profile of 24-hour digested AcV fragments with C. maculatus intestinal homogenate on chitin affinity chromatography. The column was pre-equilibrated with 0.02 M sodium tetraborate buffer, pH 7.5. The adsorbed fragments were eluted with 0.1 M HCl, in a constant flow of 1 mL/min, the absorbance was measured at 280 nm. (C) SDS-PAGE electrophoresis of the adsorbed fragments from the aforementioned chromatography: M - molecular mass marker; A. colubrina vicillin; MH - midgut homogenate of larvae; (4 to 24), time of exposure of AcV, 30 mg, to intestinal homogenate of larvae in hours in vitro. The fragments highlighted in box are more likely to be those ones found in Fig. 7 (A and C).