Table 1.
Detection of defensive proteins and biological activities throughout the AcV purification process.
| Anadenanthera colubrina |
DEFENSE PROTEINS |
||||
|---|---|---|---|---|---|
| α-amylase inhibitors | Serine inhibitors1 | Cysteine inhibitors2 | Hemolytic Activities | Lectins3 | |
| Crude extract | + | + | + | – | – |
| F3 (60–90%) | + | + | + | – | – |
| AcV | – | – | – | – | – |
| FPLC | – | – | – | – | – |
| HPLC | – | – | – | – | – |
(+) positive for activity; (-) negative for activity; the proteolytic enzymes tested, (1) serine protease (Trypsin, Chymotrypsin and Neutrophil Elastase) and (2) cysteine protease (papain); (3) Hemagglutinating activity, indicating the presence of lectins, was tested for (Type A, B, O) human erythrocytes treated and untreated with trypsin and papain.