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. 2021 Jan 20;28(9):5011–5016. doi: 10.1016/j.sjbs.2021.01.026

Table 2.

Qualitative methods for flavones in POD.

No. Test methods Qualitative features
1 Ultraviolet absorption diagram Weak spectral Ⅰ between 300 and 400 nm; strong spectral Ⅱ between 260 and 300 nm; spectral Ⅰ is acromion of spectral Ⅱ.
2 AlCl3 reaction/AlCl3 + HCl reaction When heated or placed for a long time, the solution is green, the ultraviolet fluorescence is enhanced and the absorption band is red-shifted. When hydrochloric acid is added, the absorption band is blue-shifted.
3 FeCl3 reaction Solution turns green with black precipitation.
4 HCl-Mg reaction Produce brown foam or solution turns light brown.
5 Strong alkali reaction Solution yellow deepened or turned brown.