Table 4.

Comparison of the zone of inhibition produced by different concentrations of ZnO-NPs synthesized from eucalyptus leaf extract against E. coli in well diffusion and disc diffusion method.

Treatments	Well diffusion method			Disc diffusion method
	Zone of inhibition (mm)			Zone of inhibition (mm)
	12 h	24 h	36 h	12 h	24 h	36 h
T1	3 ± 0.01j	4.5 ± 0.01i	5 ± 0.01 h	3 ± 0.01 g	4 ± 0.01e	4.5 ± 0.01d
T2	5 ± 0.01 h	5.5 ± 0.01 g	6 ± 0.02f	3.5 ± 0.01f	4.5 ± 0.012d	5.5 ± 0.02c
T3	6 ± 0.01f	7 ± 0.02e	8 ± 0.01c	4 ± 0.01e	5.5 ± 0.02c	6.5 ± 0.02b
T4	7.5 ± 0.02d	8.5 ± 0.01b	9 ± 0.02a	5 ± 0.02c	6.5 ± 0.021b	7 ± 0.025a

Whereas, T1= (10 mg/ml), T2= (30 mg/ml), T3= (60 mg/ml), T4= (90 mg/ml) concentrations of ZnO-NPs. Different letters signifies significant variations at LSD (P = 0.05), n = 3 and ± indicates standard error between three replicates.