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. 2021 Aug 9;15:709387. doi: 10.3389/fncir.2021.709387

FIGURE 4.

FIGURE 4

Depolarization of VIP neurons by ACh is consistent with an intrinsic mechanism and not presynaptic effects. (A,B) The average number of action potentials (A) and depolarization (B) elicited by an ACh puff were not affected by bath application of antagonists against AMPA, NMDA, GABAA, and glycine receptors (10 μM NBQX, 50 μM D-AP5, 5 μM SR95537, and 1 μM strychnine; action potentials: paired independence test, Z = 0.724, p = 0.52, n = 6; area: paired independence test, Z = 1.28, p = 0.26, n = 6). (C,D) The average number of action potentials (C) and depolarization (D) elicited by an ACh puff were unaffected by decreasing the ACSF Ca2+ concentration from 1.5 to 0.5 mM (action potentials: paired independence test, Z = –1.29, p = 0.25, n = 5; area: paired independence test, Z = –0.137, p = 0.94, n = 5). (A–D) Dashed horizontal lines show the mean of the control responses. Solid horizontal lines show the mean difference between the control and treatment conditions.