FIGURE 5.

α₄β₂* nAChRs do not mediate the ACh-induced depolarization of VIP neurons. (A) Example traces show that a spike train elicited by a 10 ms puff of 1 mM ACh (top) was not blocked by 10 μM DHβE (bottom), an α₄β₂* nAChR antagonist. Arrows indicate the time of the ACh puffs, and voltages indicate resting membrane potential. (B,C) DHβE did not significantly affect the average number of action potentials (B) or the total depolarization (C) elicited by ACh puffs (action potentials: paired independence test, Z = 0.185, p = 0.82, n = 5; area: paired independence test, Z = 1.40, p = 0.26, n = 5). Dashed horizontal lines show the mean of the control responses. Solid horizontal lines show the mean difference between the control and DHβE conditions.