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. 2021 Aug 9;12:709240. doi: 10.3389/fimmu.2021.709240

Figure 5.

Figure 5

The IgD-class BCR controls a rapidly responding CD21-CD23- B cell population that is able to secrete autoantibodies 24 hours after immunization. (A – E) Flow cytometric analysis of IgD-deficient (n=4/group) and WT (n=4/group) mice immunized with InsA-KLH + CpG ODN1826 or CpG ODN1826 (CI). All panels show representative plots pre-gated on lymphocytes (FSC/SSC), single cells (SSC-H/SSC-W) and viable cells (fixable viability dye, FVD-). (A) Histogram of CD19 expression used to gate B cells (CD19+) within lymphocyte gate (left). Enlarged (activated) IgM+ B cells (FSChi B220+) within B cell gate (right). (B) Histogram showing activated B cells by CD69 expression pre-gated on IgM+ B cells. (C) Representative plot showing marginal zone B cells (CD21hi CD23lo), follicular B cells (CD21lo CD23hi) and CD21- CD23- (double negative) B cell population in InsA and CI immunized IgD-deficient or WT mice. (D) Histograms of CD21-CD23- B cells IgM expression (left) and IgD expression (right). (E) Histogram showing CD23 expression in IgM+ splenic B cells.