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. 2021 Aug 9;12:709240. doi: 10.3389/fimmu.2021.709240

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Copyright © 2021 Amendt, Ayoubi, Linder, Allies, Young, Setz and Jumaa

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The CD21^- CD23^- B cell population is the major source of IgM-secreting cells under the control of the IgD-class BCR. (A–D) ELISpot analyses showing IgM secreting splenic cells of InsA-KLH + CpG ODN1826 or control (CpG ODN1826) immunized (CI) mice 24 hours after injection. IgM secretion by (A) total splenic cells, (B) CD21^-CD23^- sorted B cells, (C) CD23⁺ follicular B cells, (D) representative images of ELISpot wells of indicated cell numbers, genotypes and immunizations. Two independent experiments with n=3/group for CI and n=6/group for InsA-KLH were performed. Mean, ± SD, students t-tests with Welch’s correction was used to compare two groups within one experiment. *P < 0.05, ***P < 0.001, ****P < 0.0001.