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. 2021 Aug 9;9:676900. doi: 10.3389/fbioe.2021.676900

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Copyright © 2021 Naseri, Prause, Hamdo and Arenz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Growth assays. (A) Growth assay of yeast cells containing chromosomally integrated driver and reporter modules. (B) Growth assay of yeast cells containing chromosomally integrated driver modules. The strains containing either the integrated driver/reporter or driver modules were inoculated in induction (orange line) and non-induction (gray line) medium, followed by OD₆₀₀ measurement at 0, 6, 8, 10, 12, 24, and 36 h. Wild-type JC308 was used as a control. “1X” and “4X” indicate one or four copies of the TF binding site, respectively. To simplify the figure, the modified GAL1 promoter and CYC1 terminator located upstream and downstream of plant-derived ATFs, NLS, and the minimal CYC1 promoter located upstream of GFP are not shown. EDLLAD, activation domain from plant; GAL4AD, GAL4 activation domain of yeast; GRF9, Growth-Regulating Factor 9; JUB1, NAC TF JUNGBRUNNEN1; ANAC102, A NAC TF 102; DBD, DNA binding site; Pro, promoter. Data are expressed as the mean OD₆₀₀ values ± SD obtained from three cultures, each derived from an independent yeast colony and determined in three technical replicates. OD₆₀₀, optical density at 600 nm. The full data are shown in Supplementary Data 3.