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. 2021 Aug 9;12:684340. doi: 10.3389/fmicb.2021.684340

TABLE 2.

Comparisons of ruminal metabolites that significantly changed with yak fed diets containing different protein level ratios.

Groups
SEM q-values
Super class Metabolite names L ML MH H Treatment Linear Quadratic Cubic
Organic acids and derivatives Hydroxyurea 76,048 148,881 57,813 40,464 11,734.03 < 0.01 <0.001 0.12 0.05
4-Aminobutyric acid 10,223 29,808 35,768 29,364 3,619.94 0.06 0.36 0.32 0.02
Isocitric acid 99,404 23,365 23,365 23,365 7,452.25 < 0.001 0.01 < 0.0001 <0.0001
3-Hydroxynorvaline 528,034 842,436 304,983 189,491 71,817.96 < 0.01 <0.01 0.36 0.01
Galactonic acid 45,468 63,014 62,004 94,426 6,215.79 0.03 0.12 0.03 0.11
L-DOPA 10,989 17,795 14,423 19,832 1,157.32 0.02 0.76 0.01 0.18
2-Ketobutyric acid 105,552 144,046 103,195 98,246 5,463.85 < 0.01 <0.01 0.06 0.18
4-Acetamidobutyric acid 152,770 194,089 161,726 178,094 5,554.59 0.03 0.20 0.01 0.80
Citrulline 789,666 1,001,945 797,397 921,408 29,234.05 0.01 0.26 < 0.01 0.79
3-Hydroxybutyric acid 232,771 315,818 148,691 120,568 24,299.88 0.01 0.01 0.49 0.02
Glutaric Acid 342,226 476,562 275,466 217,889 32,762.22 0.02 0.01 0.50 0.08
N,N-dimethylarginine 103,615 175,289 79,589 42,687 17,504.52 0.04 0.01 0.58 0.15
Lipids and lipid-like molecules Citramalic acid 360,988 507,541 396,597 416,183 18,124.73 0.02 0.03 0.01 0.91
Thymol 7,920 12,635 18,864 25,635 1,930.52 < 0.01 0.04 0.06 < 0.01
Capric Acid 146,089 205,068 160,188 154,110 8,461.62 0.05 0.02 0.10 0.90
Phytanic acid 8,312 21,306 6,921 24,654 2,802.57 0.04 0.61 0.01 0.97
Arachidic acid 33,435 50,071 33,405 47,824 2,678.01 0.02 0.75 < 0.01 0.91
4-Hydroxybutyrate 40,263 49,500 24,087 23,951 3,354.78 < 0.01 0.02 0.40 0.01
Organic oxygen compounds Fucose 288,892 478,845 394,367 363,236 32,282.30 0.22 0.12 0.22 0.48
Digalacturonic acid 25,798 48,880 36,775 51,166 2,921.60 < 0.01 0.83 < 0.001 0.08
Tagatose 90,442 166,477 34,926 89,045 16,462.08 0.03 0.18 0.03 0.07
Phenylpropanoids and polyketides 3-Phenyllactic acid 298,914 341,291 304,344 83,639 30,041.52 < 0.01 0.001 0.07 0.25
Caffeic acid 10,684 22,064 15,670 19,975 1,314.10 < 0.01 0.23 0.001 0.17
Piceatannol 10,627,937 18,443,030 20,191,325 22,370,834 1425,419 0.01 0.90 0.01 0.02
Hesperitin 74,725 185,831 201,860 248,675 24,526.29 0.07 0.76 0.09 0.04
Hydrocinnamic acid 32,692,862 44,812,659 33,903,201 21,915,790 2,634,050.14 0.01 0.002 0.99 0.33
Dihydrocoumarin 64,748 96,673 43,308 23,238 9,010.95 0.02 0.01 0.70 0.05
3-(4-Hydroxyphenyl)propionic acid 43,016 70,843 53,067 30,230 4,297.77 < 0.01 <0.001 0.70 0.72
Benzenoids Pyrogallol 870,419 1,157,283 931,917 1,035,939 33,836.73 < 0.01 0.09 < 0.01 0.80
Noradrenaline 8,118 21,453 5,912 33,176 3,096.49 0.001 0.08 < 0.001 0.80
Benzoic acid 494,985 752,846 480,557 307,760 47,527.04 < 0.01 0.0006 0.57 0.15
4-Hydroxybenzoic acid 12,679 14,534 9,046 7,843 810.81 < 0.01 0.01 0.80 0.01
Organoheterocyclic compounds Maleimide 170,704 226,584 176,306 182,508 8,172.15 0.05 0.05 0.04 0.68
Urocanic acid 2,894 5,999 1,941 5,078 457.24 < 0.001 0.54 < 0.001 0.21
Others Biuret 57,008 40,288 9,128 5,786 6,325.01 < 0.01 0.21 0.31 0.001
D-Erythronolactone 93,281 116,333 83,983 97,711 3,854.94 0.01 0.12 0.01 0.12
Mannose 56,689 81,005 37,577 72,988 5,156.19 0.01 0.89 < 0.01 0.09
Atrazine-2-hydroxy 284,295 389,787 269,739 237,126 20,427.48 0.04 0.01 0.32 0.23
9-Fluorenone 410,981 538,037 407,150 481,150 20,307.76 0.06 0.32 0.01 0.68
Norleucine 12,278 20,023 13,683 11,469 1,195.13 0.04 < 0.01 0.20 0.65
1,4-Cyclohexanedione 163,682 142,916 228,063 88,456 14,010.91 < 0.001 < 0.001 0.81 0.04
Allylmalonic acid 14,826 20,897 16,422 4,032 2,077.46 0.02 < 0.01 0.38 0.45
L-Threose 361,272 641,951 339,415 203,554 55,313.76 0.03 < 0.01 0.4541 0.25
N,N-Dimethyl-p-phenylenediamine 12,025 25,724 11,245 6,745 2,302.25 0.01 < 0.01 0.24 0.22