Fig. 4.

Fermentation profiles of strain FL16 with enhancing GDP-mannose supply for improved 2′-FL production. A Concentration of total and extracellular 2′-FL, and Lactose during fermentation; B Concentration of glucose, galactose, ethanol, and cell density (OD_600nm) during fermentation. The engineered strains FL16 were cultured in YP medium containing glucose 2% (w/v) and 0.12 g/L adenine. Galactose 3% (w/v) and lactose 0.4% (w/v) were added at 24 h and cultured for another 48 h