Fig. 1.

Detection of cell population with high ALDH activity in the mouse lung. Lung cells obtained from 6-to-8-week-old wild-type C57BL/6 mice were analyzed using flow cytometry. Data were analyzed using the FACS Diva (BD Biosciences) and FlowJo (version 10.7.1, BD Biosciences) software. A ALDH^br gating determined by comparing samples stained with ALDEFLUOR alone and with ALDEFLUOR and diethylaminobenzaldehyde (DEAB), a specific inhibitor of ALDH. B–D ALDH^br cells observed in the whole lung, in the nonhematopoietic/lung-resident CD45⁻ fraction, and in the hematopoietic CD45⁺ fraction. E CD45⁻/ALDH^br cells divided further into EpCAM⁺ and PDGFRα⁺ fractions