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. 2021 Aug 23;12:471. doi: 10.1186/s13287-021-02549-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — ALDH activity in cultured cells. A ALDH activity in cultured lung CD45⁻/ALDH^br. Sorted CD45⁻/ALDH^br cells were cultured and reexamined for ALDH activity. B Surface markers on primary cultured lung fibroblasts. C ALDH activity in primary cultured lung fibroblasts. (Upper) Representative image of primary cultured lung fibroblasts stained with either ALDEFLUOR alone or ALDEFLUOR and DEAB. (Lower) Flow cytometry to determine ALDH activity in primary cultured lung fibroblasts. C ALDH activity in 3T3 and MRC fibroblast cell lines