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. 2021 Aug 9;11:689839. doi: 10.3389/fonc.2021.689839

Table 2.

The clinicopathological significance of CD44 and CD44+CD24-/low phenotype in TNBC patients.

First author, year Number of patients Studied factor Clinical significance Cut-off for considering as positive/overexpressed Evaluation method The reference ID of used antibody for IHC Number of patients with previous treatment
Diego de Mendonça Uchôa, ( 32 ) 47 CD44 It is negatively associated with lymphovascular invasion. Above 1% IHC MRQ-13 for CD44 N/A
Francesca Collina, ( 25 ) 143 CD44 It is associated with metastasis. Above 50% IHC No reference ID N/A
Weiyan Zou, ( 29 ) 51 CD44 It is associated with higher tumor grade, large tumor size, increased lymph node metastasis, and advanced tumor stage. N/A IHC ab51037 for CD44 N/A
Diego de Mendonça Uchôa, ( 32 ) 47 CD44+CD24-/low It is associated with increased tumor size. For CD44, it was 1%, and for CD24, it was 33%. IHC MRQ-13 for CD44, and SN3b for CD24 N/A
Min Hye Jang, ( 33 ) 172 CD44+CD24-/low It is associated with high tumor grade. For CD44, it was 10% and above, and for CD24, it was 10% and above. IHC 156-3C11 for CD44, and SN3b for CD24 N/A
Shu-Jyuan Chang, 2015 67 CD44+CD24-/low No statistically significant associations were found (P-values > 0.05) Not appropriately provided IHC No reference ID N/A
Fang Yang, ( 26 ) 88 CD44+CD24-/low No statistically significant associations were found (P-values > 0.05) For CD44+CD24-/low, it was above 10%. IHC ab51037 for CD44 and ab31622 for CD24 Eighty-two patients were on adjuvant chemotherapy.
Yan−Xi Liu, ( 27 ) 140 CD44+CD24-/low Compared to AR+ TNBC, it is associated with higher tumor grades. For CD44+CD24-/low, it was above 10%. IHC 156−3C11 for CD44, and Ab2−SN3b for CD24 One hundred twenty-three patients were on chemotherapy.
Hui Wang, ( 28 ) 145 CD44+CD24-/low It is positively associated with AR expression. For CD44+CD24-/low, it was above 10%. IHC ab51037 for CD44, and ab31622 for CD24 None of the patients were on targeted therapy, radiotherapy, chemotherapy, and adjuvant endocrine treatment.
Weiyan Zou, ( 29 ) 51 CD44+CD24-/low It is associated with advanced tumor stage, large tumor size, and increased lymph node metastasis. N/A IHC ab51037 for CD44 and ab31622 for CD24 N/A
Nazia Riaz, ( 35 ) 197 CD44+CD24-/low CD44+CD24-/low is correlated with the lack of AR expression. N/A IHC M7082 for CD44, and MS1279 for CD24 All of the patients were on standard radiotherapy/chemotherapy.

IHC, immunohistochemistry; N/A, not available; and AR, androgen receptor.