Table 2. Microsatellite loci characteristics and MIX assigned.
Locus | Primer (5′–3′) | Dye label | Temperature Alignment (°C) |
MIX |
---|---|---|---|---|
A9a | F: CAGGAAGGAATCTCAGGAATG R: ACGCACCGTTTCATAGTAAGG |
FAM | 58 | 1 |
A106 | F: TGGCTGATGGTATTGGTTG R: AGCACACCTTCACAGGATG |
VIC | 60 | |
B1 | F: TACAAACACATCCATCTCAGTC R: AACACTCCTATCATCCATCATC |
PET | 58 | |
C102 | F: TTCCAAACCACATTTTAGATCC R: CAGCCTTTTGATTATGGAGGT |
NED | 63 | |
A116 | F: TCGCTACTTACTCCGACCTC R: AAATCACAATGGCTTTCTCTG |
PET | 54 | 2 |
B104 | F: ACCGTAGTTGCCTGGTTACA R: AGGGTGCTGTCAGAGATGAG |
VIC | 64 | |
C105 | F: AGCAAGACCAGTTTGAAATCT R: GTTGCCCTGCGATGTAC |
NED | 58 | |
D110 | F: ATCACAAGACGAGGTTCTCAC R: GATTGGAGCAAGGGACTG |
FAM | 59 | |
B103 | F: TATTATCCACTCCTGGTCAGTC R: GTTGAAGCGTTTCCAAGAT |
FAM | 51 | 3 |