Figure 5.
Cache Valley Virus (CVV) infection of IFN-αβR-/- mice results in high in utero transmission rates and congenital abnormalities. Pregnant IFN-αβR-/- dams (CVV 4B n = 8, CVV W08491 n = 9, PBS n = 7) were challenged subcutaneously with 104 plaque forming units (PFU) of virus and (a) weight change measured daily for 5 days post infection (DPI). (b) Viremia was measured on 4 DPI. (c) Maternal organ titers for spleen, kidney, brain, liver, ovary, heart and lung, (d) pup weights, and (e) in utero tissues, including placenta and fetus brains were assayed for virus titers by plaque assays on Vero-76 cells. Each data point plotted represents the mean values and error bars indicate standard deviation. The limit of detection (LOD) is indicated with a dotted line. Statistical significance among groups were analysed by a mixed effects analysis with a Dunnett's multiple comparison test in (a,c,e), one-way ANOVA with Dunnett's multiple comparison test in (b,d), Statistically significant values are denoted by * (p < 0.05).