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. Author manuscript; available in PMC: 2021 Sep 1.
Published in final edited form as: Dev Biol. 2021 May 15;477:98–116. doi: 10.1016/j.ydbio.2021.04.009

Fig. 8. scRNA-seq comparing ECs from organoids and embryonic kidneys.

Fig. 8.

Analysis of scRNA-seq datasets of organoids and human embryonic kidney tissue from multiple publications. Organoids were differentiated using multiple protocols and ESC or PSC lines. Differentiation stages (days 0, 10, 12, 14, 16, 19, 21, 25, 26, 28, and 34 of organoid culture) do not correspond with methods used in precious figures. A) Histogram of EC percentage in organoid samples by day. B) UMAP of oECs color-coded by day. C. Heat map of 100 genes differentially expressed in eECs and oECs. Combines the top 50 genes differentially expressed in both eECs and OECs in comparison to other cells plus the top 25 genes highly expressed individually in eEC and oEC populations. Genes are grouped by cell expression similarity. Left to right, cells are grouped by gene expression similarity. eECs are indicated in green, and oECs are grey, with color coding above referring to stage. D) Differentially expressed endothelial genes in eECs and oECs.