Figure 4. SARS-CoV-2 RNA Detected in Tissues.

RT-qPCR was used to detect the presence of SARS-CoV-2 specific RNA in various tissues of deer euthanized at 4 (A) and 18 days (B) post challenge (DPC). Mean (n=2) viral RNA copy number (CN) per mg based on the nucleocapsid gene are plotted for individual animals. Colored symbols correspond to deer ID numbers: red triangles for principal infected deer and blue circles for sentinels. Asterisks (*) indicate samples with 1 out of 2 RT-qPCR reactions below the limit of detection, which is indicated by the dotted lines.