Fig. 4. Design and screening of WAp-D04-focused libraries. (a) Design of a focused library based on the WAp-D04 sequence. The library was composed of five groups of structural variations, changing consecutive amino acids according to group number and translocating the variation along the chain. X indicates a random amino acid determined by an NNK codon. (b) Sequence LOGO graphs of the final round of selection, representing the 80 most enriched sequences from the focused macrocyclic peptide library.18 Note that the first Y residue of in the LOGO graph corresponds to d-Tyr (y).
