Alignment, binding and inhibition properties of selected sequences from WAp-D04-focused, d-Tyr-initiated libraries found to bind the mWnt3a–hAFM complex. Sequences are numbered in increasing order of frequency (number of reads) within the final recovered library. Steady-state KD and IC50 values are shown for the parental and W10P single-mutant peptides. The parental WAp-D04 sequence, which appeared in the final enriched libraries as well, is also shown for comparison.
| Peptide name | Sequence | Number of reads | Steady-state KD (nM) | IC50 (nM) |
|---|---|---|---|---|
| WAp-D04 |
|
125 | 319 | 290 |
| WAp-D04-W10P |
|
8014 | 39.5 | 56 |
| WAp-D04-W10P/R2K |
|
2059 | — | — |
| WAp-D04-W10P/K3I |
|
1808 | — | — |
| WAp-D04-W10P/K3R |
|
1034 | — | — |
| WAp-D04-W10P/K3T |
|
796 | — | — |
| WAp-D04-W10P/R2K/K3V |
|
395 | — | — |