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. Author manuscript; available in PMC: 2021 Aug 23.
Published in final edited form as: Traffic. 2021 Jun 8;22(7):230–239. doi: 10.1111/tra.12804

Figure 3. SDS-PAGE analysis of synthesis and secretion of sulfated macromolecules in wild type and COG subunit KO HEK293T cells.

Figure 3

The eluates from Sephadex G-50 fine gel filtration chromatography columns were concentrated by freeze drying to contain approximately 15-20,000 cpm each in SDS-PAGE sample buffer. Parallel aliquots of each sample were subjected to no treatment (Cnt) or to treatment with cABC to degrade CS/DS (C) or hep to degrade HS and heparin (H) prior to SDS-PAGE (4-12 % gradient gels). Medium samples in panels A and B and cell lysate samples in panels C and D. Samples from COG8 deficient cells (both medium and cell lysate) in panel E.