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. Author manuscript; available in PMC: 2021 Aug 23.
Published in final edited form as: Traffic. 2021 Jun 8;22(7):230–239. doi: 10.1111/tra.12804

Figure 6. Flow cytometry with cell surface bound anti HS-antibody.

Figure 6

HEK293T cells and COG subunit deficient cell lines were suspended in PBS and incubated with the 10E4 anti-HS antibody for 30 min at 4°C before staining with goat anti-mouse IgM FITC. Controls are incubated with goat anti-mouse FITC, only. Cells were analyzed with a FACSort flow cytometer. Flow data collection was carried out with the program CellQuest 3.3 and the data generated using Kaluza Flow Cytometry analysis v 1.2. A total of 20,000 or more cells gated as viable were analyzed per sample. The figure shows data for wild type HEK293T cells (control) and the cell lines deficient in individual COG subunits 1-8.